Osteosarcoma contains a subpopulation of cancer stem-like cells that are highly resistant to radiotherapy
نویسندگان
چکیده
Methods The isolation of CSCs was performed using the sphere formation assay in serum-free medium in non-adherent conditions. The cells were characterized for the expression of mesenchymal stem cell markers (CD90/ CD105/CD73) by flow cytometry. MNNG/HOS and CSCs were irradiated with X-rays at different doses (020Gy). The sensitivity to ionizing radiation was evaluated using the MTT assay after 7 days. Cell-cycle responses were studied at 24h post-irradiation using propidium iodide staining.
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